Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Article Title: Performance of the Alere Determine ™ HIV-1/2 Ag/Ab Combo Rapid Test with specimens from HIV-1 seroconverters from the US and HIV-2 infected individuals from Ivory Coast
doi: 10.1016/j.jcv.2013.07.002
Figure Lengend Snippet: Sensitivity of assays reactivity during early HIV-1 infections relative to number of days before first positive WB. The names, abbreviations, and sources, of the HIV assays are as follows [4]: APTIMA HIV-1 Quantitative assay (Aptima, Gen-Probe, Inc., San Diego, CA); ARCHITECT® HIV Ag/Ab Combo assay (Architect; Abbott Diagnostics, Wiesbaden Germany; CE marked version was used as the US version was not available when testing was conducted); GS HIV-1/2 Combo Ag/Ab (BioRad Combo; Bio-Rad Laboratories, Redmond, WA); GS HIV-1/HIV-2 Plus O EIA (GS + O; Bio-Rad Laboratories, Redmond, WA); VITROS anti-HIV 1+2 assay (Vitros; Ortho-Clinical Diagnostics, Buckinghamshire, UK); ADVIA Centaur HIV 1/O/2 enhanced assay (Advia; Bayer, Tarrytown, NY); Abbott HIVAB HIV-1/HIV-2 (rDNA) EIA (Abbott; Abbott Laboratories, Abbott Park, IL); Avioq HIV-1 Microelisa system (Avioq; Avioq, Inc, Rockville, MD); Multispot HIV-1/HIV-2 rapid test (Multispot; Bio-Rad Laboratories); Clearview HIV-1/2 STAT-PAK (Statpak; Inverness Medical, Princeton, NJ); Clearview COMPLETE HIV-1/2 (Complete; Inverness Medical); Reveal G2 and G3 Rapid HIV-1 antibody tests (Reveal G2 or G3; MedMira Laboratories, Inc.; Halifax, Nova Scotia, Canada); OraQuick ADVANCE Rapid HIV-1/2 antibody test (Oraquick; OraSure Technologies, Inc.; Bethlehem, PA); Uni-Gold Recombigen HIV (Unigold; Trinity Biotech USA, St. Louis, MO). These assays have manufacturer reported point estimates for sensitivity ranging from 99.60% to 100.00% and point estimates for specificity ranging from 98.60% to 99.90%. The Genetic Systems HIV-1 Western blot (WB; Bio-Rad Laboratories) and Cambridge Biotech HIV-1 Western blot (WB; Maxim Biomedical Inc., Rockville, MD) have been shown to give concordant interpretations in studies conducted to qualify use in our clinical laboratory and were used interchangeably. ind: indeterminate.
Article Snippet: The specimens were classified as HIV-2 antibody positive by Multispot HIV-1/HIV-2 rapid test (Bio-Rad Laboratories, Redmond, WA) in the field and further characterized with HIV-2 WB (MP Diagnostics, The Cavendish, Singapore) at CDC.
Techniques: Western Blot